• Historical RNA expression profiles from the extinct Tasmanian tiger 

      Mármol-Sánchez, Emilio; Fromm, Bastian; Oskolkov, Nikolay; Pochon, Zoé; Kalogeropoulos, Panagiotis; Eriksson, Eli; Biryukova, Inna; Sekar, Vaishnovi; Ersmark, Erik; Andersson, Björn; Dalén, Love; Friedländer, Marc R. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-18)
      Paleogenomics continues to yield valuable insights into the evolution, population dynamics, and ecology of our ancestors and other extinct species. However, DNA sequencing cannot reveal tissue-specific gene expression, cellular identity, or gene regulation, which are only attainable at the transcriptional level. Pioneering studies have shown that useful RNA can be extracted from ancient specimens ...
    • MapToCleave: High-throughput profiling of microRNA biogenesis in living cells 

      Kang, Wenjing; Fromm, Bastian; Houben, Anna J.; Høye, Eirik; Bezdan, Daniela; Arnan, Carme; Thrane, Kim; Asp, Michaela; Johnson, Rory; Biryukova, Inna; Friedländer, Marc R. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-16)
      Previous large-scale studies have uncovered many features that determine the processing of microRNA (miRNA) precursors; however, they have been conducted in vitro. Here, we introduce MapToCleave, a method to simultaneously profile processing of thousands of distinct RNA structures in living cells. We find that miRNA precursors with a stable lower basal stem are more efficiently processed and also ...